全文获取类型
收费全文 | 12982篇 |
免费 | 1516篇 |
国内免费 | 2705篇 |
出版年
2023年 | 235篇 |
2022年 | 250篇 |
2021年 | 401篇 |
2020年 | 541篇 |
2019年 | 613篇 |
2018年 | 592篇 |
2017年 | 586篇 |
2016年 | 606篇 |
2015年 | 546篇 |
2014年 | 635篇 |
2013年 | 946篇 |
2012年 | 553篇 |
2011年 | 610篇 |
2010年 | 502篇 |
2009年 | 721篇 |
2008年 | 662篇 |
2007年 | 711篇 |
2006年 | 691篇 |
2005年 | 654篇 |
2004年 | 560篇 |
2003年 | 516篇 |
2002年 | 489篇 |
2001年 | 421篇 |
2000年 | 356篇 |
1999年 | 360篇 |
1998年 | 291篇 |
1997年 | 302篇 |
1996年 | 291篇 |
1995年 | 259篇 |
1994年 | 241篇 |
1993年 | 231篇 |
1992年 | 242篇 |
1991年 | 188篇 |
1990年 | 184篇 |
1989年 | 162篇 |
1988年 | 142篇 |
1987年 | 133篇 |
1986年 | 108篇 |
1985年 | 130篇 |
1984年 | 98篇 |
1983年 | 62篇 |
1982年 | 126篇 |
1981年 | 81篇 |
1980年 | 53篇 |
1979年 | 37篇 |
1978年 | 30篇 |
1977年 | 15篇 |
1976年 | 9篇 |
1975年 | 8篇 |
1958年 | 6篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
21.
Previous work indicated that long-term exposure to elevated carbon dioxide levels can reduce hydraulic conductance in some species, but the basis of the response was not determined. In this study, hydraulic conductance was measured at concentrations of both 350 and 700 cm3 m–3 carbon dioxide for plants grown at both concentrations, to determine the reversibility of the response. In Zea mays and Amaranthus hypochondriacus , exposure to the higher carbon dioxide concentration for several hours reduced whole-plant transpiration rate by 22–40%, without any consistent change in leaf water potential, indicating reversible reductions in hydraulic conductance at elevated carbon dioxide levels. Hydraulic conductance in these species grown at both carbon dioxide concentrations responded similarly to measurement concentration of carbon dioxide, indicating that the response was reversible. In Glycine max , which in earlier work had shown a long-term decrease in hydraulic conductance at elevated carbon dioxide levels, and in Abutilon theophrasti , no short-term changes in hydraulic conductance with measurement concentration of carbon dioxide were found, despite lower transpiration rates at elevated carbon dioxide. In G. max and Medicago sativa , growth at high dew-point temperature reduced transpiration rate and decreased hydraulic conductance. The results indicate that both reversible and irreversible decreases in hydraulic conductance can occur at elevated carbon dioxide concentrations, and that both could be responses to reduced transpiration rate, rather than to carbon dioxide concentration itself. 相似文献
22.
23.
24.
N.T. Nguyen M. Liu H. Katayama T. Takemura I. Kasuga 《Letters in applied microbiology》2021,72(3):275-282
Colistin is one of the antibiotics of last resort for human health. However, the dissemination of the plasmid-mediated colistin resistance gene mcr-1 is of great concern globally. In the One Health framework, the environment is an important component for managing antimicrobial resistance. However, little information is available concerning the prevalence of mcr-1 in water environments. We aimed to reveal the prevalence of mcr-1 in different water environments in Hanoi, Vietnam. Quantitative PCR was applied to detect mcr-1 in four urban drainages receiving untreated domestic wastewater, three rivers, five lakes and two groundwater samples. Urban drainages contained higher concentrations of mcr-1, suggesting that urban residents carry the gene. The class 1 integron-integrase gene was identified as a good surrogate of antibiotic resistance genes including mcr-1. A significant correlation was found between the levels of mcr-1 and the human-specific cross-assembly phage, which is an indicator of human faecal pollution. These results indicated that the primary source of mcr-1 in urban water environments is human faeces, which is consistent with the fact that most domestic wastewater is untreated in Hanoi. The control of untreated wastewater is critical for alleviating the spread of mcr-1 in water environments in Vietnam. 相似文献
25.
The aim of this study was to investigate the effects of the relaxation of the gluten network on pasta rehydration kinetics. The moisture content of pasta, under conditions where the effects of the diffusion of water on the moisture content were negligible, was estimated by extrapolating the average moisture content of pasta of various diameters to 0?mm. The moisture content of imaginary, infinitely thin pasta did not reach equilibrium even after 1?h of rehydration. The rehydration of pasta made of only gluten was also measured. The rate constants estimated by the Long and Richman equation for both the pasta indicated that the rehydration kinetics of infinitely thin pasta were similar to those of gluten pasta. These results suggest that the swelling of starch by fast gelatinization was restricted by the honeycomb structural network of gluten and the relaxation of the gluten network controlled pasta rehydration kinetics. 相似文献
26.
Cell division and DNA topisomerase I activity in root meristems of pea seedlings during water stress
D. CHIATANTE M. ROCCO L. MAIURO G. S. SCIPPA C. DI MARTINO J. A. BRYANT 《Plant biosystems》2013,147(3):163-173
ABSTRACT Low water potential, generated by PEG addition to the liquid medium of hydroponically grown pea seedlings, induces a fall in moisture content in the roots, followed by the arrest of elongation. This water stress reduces the mitotic index of root meristems during the treatment and induces the appearance of a peak of mitosis at 12 hours from the beginning of recovery. This peak suggests that during water stress the cell cycle is blocked in G2 or late S phase. In a first attempt to understand the biochemical events leading to cell cycle arrest, we tested the in vitro activity of DNA topoisomerase I extracted from stressed or control root meristems. The activity of this enzyme in extracts from stressed seedlings was lower than in controls, whereas it was higher in extracts from seedlings which had recovered from water stress for a few hours. The highest specific activity was observed with seedlings at 24 hours from the start of recovery. The fact that during stress treatments and recovery there was no variation in the synthesis of a 45 kDa protein, indicated as DNA topoisomerase I, suggested that the activity of this enzyme could be posttranslationally regulated. The hypothesis that variations in the concentration of unknown endogenous regulators of the activity of this enzyme may take place during water loss or uptake in the cytosol of meristematic cells is discussed. 相似文献
27.
Regulation of water flow constitutes the most important hydromorphological burden to Finnish lakes. The total area of regulated
lakes is nearly 11,000 km2, equalling one-third of the total area of Finnish inland waters. Extensive research projects have been carried out since
the end of the 1980s to find out opportunities to mitigate harmful effects of the regulation of watercourses. A water-level
fluctuation analysis tool, known as Regcel, has been developed to study water level data and to identify the most significant
impacts. Results of the Regcel analysis give an overall picture of the impact of lake regulation in northern climate. The
model is based on relationships between the water-level fluctuation and factors related to environmental, social and economical
effects. Regcel has been used in 12 Lake Regulation Development Projects in Finland. In this article, we show how the Regcel
model was applied in two cases.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users.
Guest editors: K. M. Wantzen, K.-O. Rothhaupt, M. M?rtl, M. Cantonati, L. G.-Tóth & P. Fischer
Ecological Effects of Water-Level Fluctuations in Lakes 相似文献
28.
Photosynthesis,Transpiration, and Water Use Efficiency of Vegetative and Reproductive Shoots of Grassland Species from North-Eastern China 总被引:2,自引:0,他引:2
The differences in net photosynthetic rate (P
N), transpiration rate (E), and water use efficiency (WUE) between the vegetative and reproductive shoots of three native grass species from the grassland of northeastern China [grey-green and yellow green populations of Leymus chinensis (Trin.) Tzvel., Puccinellia tenuiflora (Griseb) Scrib & Merr, Puccinellia chinampoensis Ohwi] were compared. The two type shoots experienced similar habitats, but differed in leaf life-span and leaf area. The leaf P
N and WUE for the vegetative shoots were significantly higher than those for the reproductive shoots in the grasses, while their E were remarked lower in the dry season. Relative lower leaf P
N and WUE for the reproductive shoots of grassland grasses may explain the facts of lower seed production and the subordinate role of seed in the grassland renewal in north-eastern China. 相似文献
29.
Effect of soil water stress on soil respiration and its temperature sensitivity in an 18-year-old temperate Douglas-fir stand 总被引:5,自引:0,他引:5
RACHHPAL S. JASSAL T. ANDREW BLACK MICHAEL D. NOVAK DAVID GAUMONT-GUAY ZORAN NESIC 《Global Change Biology》2008,14(6):1305-1318
We analyzed 17 months (August 2005 to December 2006) of continuous measurements of soil CO2 efflux or soil respiration (RS) in an 18‐year‐old west‐coast temperate Douglas‐fir stand that experienced somewhat greater than normal summertime water deficit. For soil water content at the 4 cm depth (θ) > 0.11 m3 m?3 (corresponding to a soil water matric potential of ?2 MPa), RS was positively correlated to soil temperature at the 2 cm depth (TS). Below this value of θ, however, RS was largely decoupled from TS, and evapotranspiration, ecosystem respiration and gross primary productivity (GPP) began to decrease, dropping to about half of their maximum values when θ reached 0.07 m3 m?3. Soil water deficit substantially reduced RS sensitivity to temperature resulting in a Q10 significantly < 2. The absolute temperature sensitivity of RS (i.e. dRS/dTS) increased with θ up to 0.15 m3 m?3, above which it slowly declined. The value of dRS/dTS was nearly 0 for θ < 0.08 m3 m?3, thereby confirming that RS was largely unaffected by temperature under soil water stress conditions. Despite the possible effects of seasonality of photosynthesis, root activity and litterfall on RS, the observed decrease in its temperature sensitivity at low θ was consistent with the reduction in substrate availability due to a decrease in (a) microbial mobility, and diffusion of substrates and extracellular enzymes, and (b) the fraction of substrate that can react at high TS, which is associated with low θ. We found that an exponential (van't Hoff type) model with Q10 and R10 dependent on only θ explained 92% of the variance in half‐hourly values of RS, including the period with soil water stress conditions. We hypothesize that relating Q10 and R10 to θ not only accounted for the effects of TS on RS and its temperature sensitivity but also accounted for the seasonality of biotic (photosynthesis, root activity, and litterfall) and abiotic (soil moisture and temperature) controls and their interactions. 相似文献
30.
After E. coli cells (WP2 and WP2uvrA) were treated with chemical mutagens (methyl methanesulfonate, MMS; N-methyl-N-nitrosourea, MNU; 4-nitroquinoline 1-oxide, 4NQO) in 1/15 M phosphate buffer, the mutability of the treated cells plated on a D2O-agar plate was compared with that plated on an ordinary H2O-agar plate. The mutation frequency decreased more or less on the D2O-agar plate. The D2O-substitution effects, as termed by the relative mutation frequencies (MFD2O/MFH2O), are 0.92 for MMS, 0.29 for MNU, and 0.42 for 4NQO in WP2, and 0.68 for MMS, 0.49 for MNU, and 0.16 for 4NQO in WP2uvrA. The D2O effect seemed to be partly related to the function of the uvrA gene-associated products. The pH dependence of mutability was discussed in connection with the D2O-substitution effect. 相似文献